DescriptionViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is next generation first choice mix for a convenient and rapid one step real-time PCR reaction set up. The master mix has been freeze-dried to produce a room temperature stable preparation. The freeze-dried master mix contains unique thermostable M-MULV enzyme, Taq DNA polymerases, MgCl2 and buffer components at optimal concentration. ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix has same optimal performance as ViPrimePLUS One Step qRT-PCR Master Mix and ViPrimePLUS One Step qRT-PCR Green Master Mix. The lyophilized qRT-PCR Master Mix can detect extremely low copy number targets of RNA templates in one step complete system. The removal of separate reverse transcription step not only reduces errors and time to obtain results, but also leads the qRT-PCR reaction to optimum sensitivity and specificity. ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is used with supplied re-suspension buffer and ROX dye; hence hardware platforms that need ROX as passive reference dye can be used in standard cycling conditions.

Features

• Convenient and rapid real time PCR reaction set up
• Lyophilized form – room temperature stable preparation
• Equipped with thermostable M-MULV enzyme and Taq DNA Polymerase
• Reliable quantification of low-abundance transcripts very specifically with high efficiency
• Compatible with most of the real-time PCR platforms

Kit Component

• 3 x Lyophilized qPCR MasterMix
• 1 x Lyophilized ROX
• 4 x Re-suspension buffer

Master Mixes Selection Guidelines for System CompatibilityTo calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.

Real-time PCR platforms	ROX re-suspension volume	ROX addition per tube of master mix
Applied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers.	100µl	20µl
All Stratagene platforms	200µl	15µl
Applied Biosystems 7500 platform	700µl	10µl
All other machines	Not Required	Not Required

Ordering Information

Catalog No	Description	Pack Size
QLMM06	ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix	150 reactions

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ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。