DescriptionThe ViPrimePLUS Ovies aries (Sheep) qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Ovies aries (Sheep) genome in food samples.ViPrimePLUS Ovies aries (Sheep) qPCR Kit is intended for research use only.

Kit Component

• Sheep Primers and Probe Mix (Sheep PPM)
• Positive Control
• Internal Extraction Control Primers and Probe Mix (IEC PPM)
• Internal Extraction Control (IEC DNA)
• Nuclease Free Water
• Template Preparation Buffer

Product Specification

Technology	Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit	DNA
Kit Storage	Shipped in room temperature, store at -20℃ on arrival
Sample Material	Food samples
Gene Target	Mitochondrial DNA specific to the species
Detection Limit	10 to <100 copies per reaction
Sensitivity & Specificity	Ct value 28±3 with probability of 95%
Controls Included	•Internal Extraction Control (IEC) as inhibition control •Positive control
Channels	•FAM channels detect pathogen amplicons•VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No	Description	Pack Size
QI0004	ViPrimePLUS Ovies aries (Sheep) qPCR Kit	100 reactions

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ViPrimePLUS Ovies aries (Sheep) qPCR Kit

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。