Concentration5-20u/μl5"…CCG↓CTC…3" 3"…GGC↑GAG…5"

Reaction Conditions 1X Buffer V5 30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes

Ligation / Recutting Assay After 5-fold overdigestion with AccBSI, 90% of the DNA fragments can be ligated and of these 50% can be recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccBSI for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Catalog No	Description	Pack Size
RE1110	AccBSI {BsrBI}	500u

 

DownloadsManualAccBSI {BsrBI}

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。