MutaGEL r-Vitamin D3 PCR Assay

MutaGEL r-Vitamin D3 PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only

Controls Included

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Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “VD3R positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).

Assay Principle

The MutaGEL® r-vitamin D3 PCR Assay contains a set of primers for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of varying genotypes (start codon polymorphism) is characterized by subsequent specific restriction enzyme digestion. The rare variant (f, pathogenic) possesses a restriction site for the specific endonuclease, whereas the major variant (F, protective) does not. The amplified products obtained from the pathogenic DNA variant will, therefore, be cut into two fragments, whereas the protective DNA variant will not be cut. The identification of the existing genotype from the patient is done by subsequent analysis of the DNA fragments by gel-electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).

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Learn more about Vitamin D at Eagle Biosciences Biomarker Spotlight page dedicated to Vitamin D here:

Vitamin D Biomarker Spotlight

EagleBio的iLite®分析就绪细胞iLite®  技术基于报告基因检测格式，经过修改和适用于整个药物开发周期中的应用以及生物药物的监测。这些细胞系可以开发用于任何生物制药靶标，并且可以使用相同的细胞系轻松设置药物效力（即药物活性）的测定方法。检验就绪的细胞经过基因工程处理，可与报告基因检验技术一起使用，以检测药物效力和NAb。