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Recombinant Human Phospho-Ubiquitin (S65) Protein, CF Summary
Reaction conditions will need to be optimized for each specific application.
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
U-102
| Formulation | X mg/ml (X μM) in 10 mM HEPES pH 7.5 |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Ubiquitin
Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.
Recombinant Phospho-Ubiquitin (pS65) is generated enzymatically using PINK1 kinase from Red Flour Beetle (Tribolium castaneum).
- Matsuda N. et al. (2010) J. Cell Biol. 189: 211
- Kane L.A. et al. (2014) J. Cell Biol. 205:143
- Ordureau A. et al. (2014) Mol Cell. 56: 360
- Vives-Bauza C. et al. (2010) Proc. Natl. Acad. Sci.) 107: 378
- Wall C.E. et al. (2019) Cell Reports 29: 3280
- Wauer T. et al. (2015) EMBO J. 34: 307
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