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蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组人UBE2I/Ubc9蛋白，CF/E2-645-100

波士顿生物化学/重组人UBE2I/Ubc9蛋白，CF/E2-645-100

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￥4780.00
货号：E2-645-100
浏览量：127
品牌：Boston Biochem
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Citations (7)
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human UBE2I/Ubc9 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human UBE2I/Ubc9 is a member of the SUMO-conjugating (E2) enzyme family that receives SUMO from a SUMO-activating (E1) enzyme and subsequently conjugates SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2I/Ubc9 protein
Accession #
P63279
Predicted Molecular Mass
18 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-645

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.

ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2I/Ubc9

Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins (1,2). SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9 (3). In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition (4). UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML (5,6). Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition (5,7). UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance (8). SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers (9).

References
  1. Desterro, J.M. et al. (1999) J. Biol. Chem. 274:10618.
  2. Johnson, E.S. & G. Blobel (1997) J. Biol. Chem. 272:26799.
  3. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  4. Yunus, A.A. & C.D. Lima (2006) Nat. Struct. Mol. Biol. 13:491.
  5. Knipscheer, P. et al. (2008) Mol. Cell 31:371.
  6. Duprez, E. et al. (1999) J. Cell Sci. 112:381.
  7. Su, Y.F. et al. (2012) PLoS One 7:e34250.
  8. Wu, Y. et al. (2012) PLoS One 7:e39606.
  9. Mo, Y.Y. et al. (2005) Oncogene 24:2677.
Additional References
  1. Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
  2. Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
  3. Liu Q., et al. (1999) J. Biol. Chem. 274:16979-16987
  4. Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
  5. Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
  6. Tatham M.H., et al. (2003) Biochem. 42:3168-3179
  7. Tong H., et al. (1997) J. Biol. Chem. 272:21381-21387
  8. Watanabe T.K., et al. (1996) Cytogen. Cell. Genet. 72:86-89
  9. Yasugi T and Howley P.M (1996) Nuc. Acid. Res. 24:2005-2010
Long Name
Ubiquitin-conjugating Enzyme E2I
Entrez Gene IDs
7329 (Human); 22196 (Mouse)
Alternate Names
C358B7.1; EC 6.3.2; EC 6.3.2.-; p18; SUMO-1-protein ligase; SUMO-protein Ligase; Ubc9; UBC9SUMO-conjugating enzyme UBC9; UBCE9; UBE2I; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

Citations for Recombinant Human UBE2I/Ubc9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

7Citations: Showing 1 - 7Filter your results:

Filter by:

  1. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFbAuthors: X Ma, T Yang, Y Luo, L Wu, Y Jiang, Z Song, T Pan, B Liu, G Liu, J Liu, F Yu, Z He, W Zhang, J Yang, L Liang, Y Guan, X Zhang, L Li, W Cai, X Tang, S Gao, K Deng, H ZhangElife, 2019;8(0):.Species: HumanSample Types: Recombinant ProteinApplications: SUMOylation Assay
  2. Mechanism of Adenovirus E4-ORF3-Mediated SUMO ModificationsAuthors: SY Sohn, P HearingMBio, 2019;10(1):.Species: HumanSample Types: Recombinant ProteinApplications: Enzyme Assay
  3. Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesionAuthors: S Appikonda, KN Thakkar, PK Shah, SYR Dent, JN Andersen, MC BartonJ. Biol. Chem., 2018;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay
  4. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cellsAuthors: WF Wang, L Yan, Z Liu, LX Liu, J Lin, ZY Liu, XP Chen, W Zhang, ZZ Xu, T Shi, JM Li, YL Zhao, G Meng, Y Xia, JY Li, J ZhuNat Commun, 2017;8(1):363.Applications: Bioassay
  5. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J LuoBMC Mol. Biol., 2017;18(1):23.Species: N/ASample Types: ProteinApplications: Bioassay
  6. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J LuoBMC Mol. Biol., 2017;18(1):23.Species: HumanSample Types: Whole CellsApplications: Bioassay
  7. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongationProc Natl Acad Sci USA, 2016;113(24):6725-30.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

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Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。