INFORMATION: The FOXP3 molecule is a 50–55 kD transcription factor also known as IPEX, JM2, Forkhead box 3, and Scurfin. Defects in this gene can result in lethal autoimmune disease (2). When used with cell surface markers CD4 and CD25(IL-2R), FOXP3 is a useful marker for identifying T regulatory cells. Functionally, FOXP3 is thought to mediate oxidative phosphorylation, enabling Treg to function in a lower oxygen environment (3).

In EIA, clone ANCFX2D7 binds to full length recombinant FOXP3 and to a FOXP3(R1-R3) construct (aa 1–198) but not to a FOXP3(R1) construct (aa 1-70), suggesting that its epitope is within regions 2 and 3 (aa 71–198).

ANCFX2D7 binds to nuclear FOXP3 found constitutively in a 0.5 – 3% sub population of fixed, permeabolized CD4+ peripheral blood lymphoid cells in FACS.

FOXP3 References:

1) G Roncador, A H Banham, et al. (2005) Eur J Immunol 35(6): 1681-1691. PMID: 15902688.

2) S Hori, S Sakaguchi, (2004) Microbes and Infection 6(8): 745-751.

3) U H Beier, W W Hancock, et al. (2016) J Immunol 196(1 suppl 211.3).

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。