Description

Product Name	Tetramethylrhodamine - 5 - maleimide
Size	5 mg
Catalog #	AS-81446
US$	$254
Maleimides are among the most frequently used reagents for thiol modification.In most proteins, the sites of reactions are at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Tetramethylrhodamine-5-maleimide is widely used for thiol modifications of peptides and proteins.
Detailed Information	Material Safety Data Sheets (MSDS)
Storage	-20°C desiccated and protected from light
References	1. Graceffa P and Dominguez R (2003).J Biol Chem 278, 34172-80. 2. Tong Y, et al. (2001).J Gen Physiol 117, 103-18.
Molecular Weight	481.499
Molecular Formula	C28H23 N3O5
Spectral Properties	Abs/Em = 540/567 nm
Solvent System	DMF or DMSO
Product Citations	Iwamoto, M. & S. Oiki (2013). Amphipathic antenna of an inward rectifier K+ channel responds to changes in the inner membrane leaflet. PNAS 110, 749. doi: 10.1073/pnas.1217323110.Srinivasan, S. et al. (2011). L-selectin transmembrane and cytoplasmic domains are monomeric in membranes. Biochim Biophys Acta-Biomembranes doi:10.1016/j.bbamem.2011.02.006.Bakker, A. et al. (2008). Conditional MHC class I ligands and peptide exchange technology for the human MHC gene products HLA-A1, -A3, -A11, and -B7. PNAS 105, 3825.

Anaspec在过去的二十年中，荧光检测生理学和神经学信号受到了极大的关注。使用分子荧光或发光来感测生理和神经信号具有若干优点。其中包括高灵敏度（低至单个分子），开-关切换性，活细胞通信的可行性，具有亚微米可视化的亚纳米空间分辨率和亚毫秒级时间分辨率。光诱导电子转移（PET）和电子能量转移（EET）主要用于将离散的和化学计量的识别事件转换为荧光信号。基于PET效应，开发了许多荧光生理指示剂（如下所述）。AnaSpec提供高质量和具有竞争力的价格的多种生理和神经指标（例如Fura-2，Indo-1和Rhod-2），用于各种生物学应用。AnaSpec努力提供可在任何地方使用的最高纯度的指标。通过HPLC分析，我们的UltraPure级AM荧光指示剂酯的纯度通常至少为95％，尽管纯度通常超过98％。此外，我们还为您提供许多Ca2 +指示剂的AM酯的定制包装服务，以最小的费用为您提供便利，并降低了存储期间变质的风险。