Anaspec/MBP,MAPK底物,生物素化,磷酸化/1mg/AS-29877

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¥5080.00
货号:AS-29877
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品牌:Anaspec
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商品描述
DescriptionSequence(One-Letter Code)
Product NameMBP, MAPK Substrate, Biotinylated, PhosphorylatedBiotin - APR - pT - PGGRR
Size1 mg
Catalog #AS-29877
US$$254
Purity% Peak Area By HPLC ≥ 95%

This is an N-terminally biotinylated peptide, with a phosphorylated Thr97. The sequence APRTPGGRR contains a native sequence derived from bovine myelin basic protein amino acids 95-98 (PRTP). The rest of the sequence is not derived from a native sequence, but is a synthetic construct. APRTPGGRR is specific for MAP kinases: p44MAPK [extracellular signal-regulated kinase 1 (ERK1)] and p42MAPK (ERK2). It contains the consensus sequence Pro-X-(Ser/Thr)-Pro that is recognized by MAP kinase. APRTPGGRR is the most efficient substrate for phosphorylation reaction by ERK and is phosphorylated by kinases on threonine 97 and can also be phosphorylated by MAPK p38.

Detailed InformationMaterial Safety Data Sheets (MSDS)
Storage-20°C
ReferencesRef: Sommer, F. et al. Int. J. Impot. Res. 14, 217 (2002); Lents, NH. et al. Ann. N. Y. Acad. Sci. 973, 265 (2002); Kameshita, I. et al. J. Biochem. (Tokyo) 122, 168 (1997).
Molecular Weight1273.4
Biotin-APR-pT-PGGRR
Sequence(Three-Letter Code)Biotin - Ala - Pro - Arg - pThr - Pro - Gly - Gly - Arg - Arg - OH
Anaspec在过去的二十年中,荧光检测生理学和神经学信号受到了极大的关注。使用分子荧光或发光来感测生理和神经信号具有若干优点。其中包括高灵敏度(低至单个分子),开-关切换性,活细胞通信的可行性,具有亚微米可视化的亚纳米空间分辨率和亚毫秒级时间分辨率。光诱导电子转移(PET)和电子能量转移(EET)主要用于将离散的和化学计量的识别事件转换为荧光信号。基于PET效应,开发了许多荧光生理指示剂(如下所述)。AnaSpec提供高质量和具有竞争力的价格的多种生理和神经指标(例如Fura-2,Indo-1和Rhod-2),用于各种生物学应用。AnaSpec努力提供可在任何地方使用的最高纯度的指标。通过HPLC分析,我们的UltraPure级AM荧光指示剂酯的纯度通常至少为95%,尽管纯度通常超过98%。此外,我们还为您提供许多Ca2 +指示剂的AM酯的定制包装服务,以最小的费用为您提供便利,并降低了存储期间变质的风险。