Name

HiFiScript 1st Strand cDNA Synthesis Kit

Cat. #

Application

• Reverse transcription, 1st strand cDNA synthesis
• cDNA library construction

Specifications

HiFiScript 1st Strand cDNA Synthesis Kit has been designed for the first-strand cDNA synthesis kit in a two-step RT-PCR experiments. HiFiScript reverse transcriptase in the kit is a new recombinant Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with novel mutations, which endorse HiFiScript the following features:

• High transcriptional efficiency – higher yields of cDNA
• High sensitivity – pg level total RNA and/or mRNA can be used to synthesis first strand cDNA.
• RNase H activity is removed – improved the elongation ability of the enzyme, and increased affinity of transcriptase to RNA template.Generate first strand cDNA up to 12 kb.

The optimized RT Buffer make the reverse transcriptase a wider range of applications, and with high compatibility for subsequent PCR and quantitative PCR experiments.

Shipping / Storage

Manual (protocol)

RT reaction system

Note: If the starting RNA is less than 50 ng, RNase Inhibitor (RNasin, Cat.#: W0596) should be added.