快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / 101Bio / 101Bio/3D肿瘤培养凝胶/2 mL/P720S-2

101Bio/3D肿瘤培养凝胶/2 mL/P720S-2

价格
面议
货号：P720S-2
浏览量：127
品牌：101Bio
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Home »products » Cell Biology Product » 3D Tumor Culture Gel
3D Cell Culture Gel - Col-Tgelfor tumor cell, stem cell and primary cell
Col-Tgel Flyer
Cat.#StiffnessVolumeStrength3 Stiffness Application
P720S-2Soft2 mL0.9 ~ 1.5 kPaCirculating cells, Nerve cells, BT474, HL60, P39
P720S-10Soft10 mL
P720M-2Med2 mL14 ~ 20 kPaMuscle cells, Adipose cells, C2C12, HepG2
P720M-10Med10 mL
P720H-2Stiff2 mL35 ~ 47 kPaBone cells
P720H-10Stiff10 mL
P720SMH-6S/M/H2 mL x 3A combo of P720S-2, P720M-2, P720H-2 for customer testing their cells.
Customer can mix any two gels at any ratio to make their own special stiffness gel.
How to pay with
Also can buy from :
P720SMH-6, Fisher Cat.# NC1291700

Application

Col-Tgel is a tailorable 3D cell culture matrix for tumor cell, stem cell and primary cell. We provide soft, medium and stiff gel for your different cell types.

It provides collagen backbone for cell attachment and allows customizing matrix components by additional extracellular matrix, growth factors, cytokines, and/or hormones for optimizing three dimensional cell culture.

This product is for research use only.

Stiffness selection for different cell types

3D Cell Culture Gel - 3 stiffness

Click to see full size image.

Formation of tumor spheroids in Col-Tgel

Col-Tgel for tumor cell, stem cell and primary cell

Fang, JY., etc. PloS one 9, no. 8 (2014): e105616.

For immunohistochemistry and immunocytochemistry method, please refer to Page 2 of this publication.

Unique Features

  • Handle at room temperature (no need on ice)
  • Save money: 30% below market price
  • Save time: No hanging drop, rotating wall vessel bioreactors
  • 3 stiffness(unique feature): to fit most cell types
  • 2 mL aliquotes: avoid freeze-thaw cycle - user friendly
  • Cytokine / Hormon free: no background
  • Flexible:

    -Col-Tgel can be customized for different experiments.- Geometry: 3D embedding, 2D seeding on the top of hydrogel, or unique molds.
    -Sample size and quantity: 6- to 96- well with/without tissue culture insert or transwell. - Matrix component and stiffness: collagen-based with different yield strength (i.e. Soft, Med and Stiff).
    - Transparency: easy to observe directly under microscopes and can apply with immunohistochemistry directly.

    • Col-Tgel is a tailorable collagen-based, cell remodelable hydrogel system. It not only can be used a carrier for cell or drug delivery, but also in vitro cell culture platforms. Besides, it contains cell essential nutrients, and provides a stable and durable platform for long-term studies such as cell-cell, cell-matrix interactions, and etc.
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, easy handling

Shipping / Storage

Ship at room temperature.Store Component A (gel) at 4oC, and store Component B (crosslinker) at -80oC for 9 months shelf life, or at -20oC for 3 months, or at 4oC for 1 month.Avoid freeze-thaw cycle for Component B.

Shelf Life

Component A: 12 months at 4oC

Component B: 9 months at -80oC; 3 months at -20oC; 1 month at 4oC

Manual (protocol)

101Bio.com 3D Cell Culture Gel - Col-Tgel

Citations

Citation 1:
Kuwahara, Kenrick, Zhi Yang, Ginger C. Slack, Marcel E. Nimni and Bo Han.Cell delivery using an injectable and adhesive transglutaminase–gelatin gel.Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618.
Citation 2:
Kuwahara, Kenrick, Josephine Y. Fang, Zhi Yang and Bo Han.Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity. Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964.
Citation 3:
Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata and Bo Han.Injectable gel graft for bone defect repair.Regenerative medicine 9, no. 1 (2014): 41-51.
Citation 4:
Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni and Bo Han. The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation.Biomaterials 35, no. 20 (2014): 5294-5306.
Citation 5:
Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag and Bo Han. Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel.PloS one 9, no. 8 (2014): e105616.
Citation 6:
Shih-Jye Tan, Fang, Josephine Y., Y. Wu, Zhi Yang, G. Liang and Bo Han.Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation.Sci Rep. 2015 Nov 9;5:16333. doi: 10.1038/srep16333.
Citation 7:
Lee G, Kim HJ, Kim HM.RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells.J Dent Res. 2015 Dec 3. pii: 0022034515619375. [Epub ahead of print]
Citation 8:
Fang JY, Tan SJ, Wu YC, Yang Z, Hoang BX, Han B.From competency to dormancy: a 3D model to study cancer cells and drug responsiveness.J Transl Med. 2016 Feb 4;14(1):38. doi: 10.1186/s12967-016-0798-8.
* Different names (TGase gel, TG gel) were used in old publications.They referred to the same 3D cell culture gel, and we finally name it as Col-Tgel.
See all 101Bio"s citation papers, click here
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, easy handling
3D Cell Culture Gel - Col-Tgel for tumor cell, stem cell and primary cell, in vitro and in vivo
Tumor and regular tissue progression and development in Col-Tgel for in vitro and in vivo application.
Exosome IsolationPurity > 95%
Protein Extraction1 min total protein, 40 min membrane protein
3D Cell Culture Gel30% < mkt="">
PCR Kits50% < mkt="">
Mouse Brain Microvessel Isolation KitEasy protocol and high yield
Endotoxin-Free Plasmid Kitsmaxi, midi and mini-prep
101Bio外泌体服务外泌体分离服务   （从细胞培养基，血清，尿液和其他体液样本中分离）我们可以为您分离纯的外泌体。将您的细胞或其他形式的样品（例如血清，尿液等）发送给我们，我们将向您发送95％纯的外泌体。外泌体RNA和蛋白质提取服务我们从通过PureExo试剂盒纯化的外泌体中提取高纯度的总RNA（包括小RNA片段）。我们可以提取有或没有去污剂的总外泌体蛋白。疾病细胞/人血清外泌体分离服务    链接我们可以为您从以下疾病细胞中提取纯的外泌体：急性骨髓性白血病（AML）人骨髓单核细胞慢性粒细胞白血病，费城阳性（CML +）人骨髓单核细胞慢性粒细胞白血病，费城阴性（CML-）人骨髓单核细胞急性淋巴细胞白血病（ALL）人骨髓单个核细胞非霍奇金淋巴瘤（NHL）人骨髓单核细胞淋巴增生综合征（LPS）人骨髓单核细胞多发性骨髓瘤（MM）人骨髓单核细胞骨髓增生异常综合症（MDS）人骨髓单个核细胞浆细胞瘤（PC）人骨髓单核细胞再生障碍性贫血（AA）人骨髓单核细胞自身免疫性溶血性贫血（AHA）人骨髓单核细胞牛皮癣（Ps）人类皮肤细胞类风湿关节炎（RA）人类皮肤细胞类风湿关节炎（RA）人牙髓细胞系统性红斑狼疮（SLE）人类皮肤细胞外泌体荧光标记用荧光染料染色总外泌体。外泌体抗体标记为了识别/追踪目的，用荧光偶联抗体标记外泌体。外泌体FACS分类我们可以使用流式细胞仪对特定的外泌体进行分类，以供您研究。