Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell migration, cytokinesis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo.

For preparation of Ser-3 phosphorylated Cofilin, purified human Cofilin 1 was phosphorylated in vitro with His-tagged mouse recombinant LIM Kinase 1 for 30 min. at 37°C (Cat. #CL7321). For control lysate, the recombinant Cofilin 1 protein was exposed to buffer only (Cat.#CL7311). The phosphorylated product is detected by anti-Cofilin (Ser-3; Cat.#CP1151), while both the control and phosphorylated recombinant cofilin 1 are detected by anti-Cofilin (N-terminus; Cat.#CP1131).

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