StreptavidinAlexa Fluor^®647 Monovalent Antibody Labeling Kit ab272190 is used to label biotinylated antibodies withAlexa Fluor^®647.

The Antibody - StreptavidinAlexa Fluor^®647 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.

Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.Use this kit to:- label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody

- label two or morebiotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment*multiple kits required, see list below

- label as little as 0.1 µg of biotinylated antibodyin less than 5 mins without washes

Technical notesThe 50µg size of this kit is typically sufficient to label 50µg of antibody, the 25 µg size is sufficient to label25 µgof antibody.

When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.

The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.

List of available monovalent streptavidin antibody labeling kits

Streptavidin Alexa Fluor^®488 (ab272187)Streptavidin Atto 542 (ab272188)Streptavidin Alexa Fluor^®594 (ab272189)Streptavidin Alexa Fluor^®647 (ab272190)– this kit

Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies.