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蚂蚁淘在线 / 品牌中心 / Abcam / Abcam/重组人EGF蛋白（无动物）（ab9697）/ab9697

Abcam/重组人EGF蛋白（无动物）（ab9697）/ab9697

价格
面议
货号：ab9697
浏览量：127
品牌：Abcam
服务
全国联保
正品保证
正规发票
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商品描述

Description

  • Product name

    Recombinant human EGF protein (Animal Free)See all EGF proteins and peptides

  • Biological activity

    The ED50,as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

  • Purity

    >= 98% SDS-PAGE.Sterile filtered.Greater than 98% pure by HPLC analyses.

  • Endotoxin level

    <>

  • Expression system

    Escherichia coli

  • Accession

    Q6QBS2

  • Protein length

    Full length protein

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

    • Predicted molecular weight

      6 kDa

    • Amino acids

      971 to 1023

    • Additional sequence information

      ab9697 is a globular protein containing 53 amino acid residues, including 2 intramolecular disulfide bonds.

Associated products

  • Related Products

    • Anti-EGF antibody (ab9695)

Specifications

Our Abpromise guarantee covers the use ofab9697in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Cellular Activation

    SDS-PAGE

    HPLC

  • Form

    Lyophilized

  • Additional notes

    Manufactured using all non-animal reagents.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.

General Info

  • Alternative names

    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.

  • Involvement in disease

    Hypomagnesemia 4

  • Sequence similarities

    Contains 9 EGF-like domains.Contains 9 LDL-receptor class B repeats.

  • Post-translationalmodifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

  • Cellular localization

    Membrane.
  • Information by UniProt
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