Abcam/人Th1/Th2/Th17抗体阵列-膜(34靶点)(ab169809)/1 x 4膜/34靶点(ab169809)

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货号:34targets)(ab169809
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品牌:Abcam
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商品描述

Overview

  • Product name

    Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets)See all Cytokine Antibody Array antibody arrays
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with:Human
  • Product overview

    ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.

    Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.

    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair ofspots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

    Learn more about membrane antibody arrays

  • Notes

    A table listing all of ourhuman membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for:Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components1 x 4 Membranes1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin1 x 50µl1 x 50µl
    1X Blocking Buffer1 x 25ml2 x 25ml
    2000X Biotin-Conjugated Anti-Cytokines1 x 2 vials1 x 4 vials
    20X Wash Buffer I1 x 10ml1 x 20ml
    20X Wash Buffer II1 x 10ml1 x 20ml
    2X Cell Lysis Buffer1 x 10ml1 x 16ml
    8-Well Incubation Tray (with Lid)1 unit1 unit
    Angiogenesis Antibody Array Membranes4 units8 units
    Detection Buffer C1 x 1.5ml1 x 2.5ml
    Detection Buffer D1 x 1.5ml1 x 2.5ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Antibody Arrays
    • Membrane antibody arrays
    • Cytokine antibody arrays
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