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Overview
Product name
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets)See all Cytokine Antibody Array antibody arraysSample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture mediaAssay type
Semi-quantitativeSpecies reactivity
Reacts with:MouseProduct overview
ab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.
Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGF
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
Notes
If you are interested in this cytokine array, a tableof mouse cytokine membrane arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applications
Suitable for:Multiplex Protein Detectionmore details
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial Cytokine Antibody Array Membranes 4 units 2 x 4 units 2 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Research areas
- Immunology
- Innate Immunity
- Cytokines
- Interleukins
- Immunology
- Innate Immunity
- Cytokines
- Interferons
- Antibody Arrays
- Membrane antibody arrays
- Cytokine antibody arrays
