The FirePlex^®miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex^®Analysis Workbench software.

Our multiplex miRNA assays use our FirePlex^®particle technology to profile up to 65 miRNA directly from 5 micron sections of FFPE or fresh-frozen tissue, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex^®hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.

The FirePlex^®miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay"s multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 5 micron of tissue sample.

For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-assays/fireplex-mirna-assays