Recommended control: Human Wild Type A549 cell line (ab255450)

Please note a wild type cell line is not automatically included with a KO cell line order, if required please add recommended wild type cell line at no additional cost using the code WILDTYPE-TMTK1

Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, supplemented with 10% (v/v) DMSO

Handling procedure: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C will result in loss of viability.1. Thaw the vial in 37°C water bath approximately 1-2 minutes.2. Transfer the cell suspension to a 15 mL conical tube with pre-warmed 5 mL complete mediumDMEM+10% FBS, spin 125×g for approximately 5 minutes at room temperature.3. Resuspend the cell pellet with 1 mL pre-warmed complete mediumDMEM+10% FBS and dispense into a 25cm² culture flask containing 10 mL pre-warmed complete complete mediumDMEM+10% FBS.4. Incubate the culture at 37°C incubator with 5% CO₂.5. A subcultivation ratio of 1:4-1:6 is recommended. Cells should be passaged when cells grow splitting at 80-90% confluence.

Click here to view the Mammalian cell tissue culture protocol

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.