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蚂蚁淘在线 / 品牌中心 / Abcam / Abcam/链霉亲和素（HRP）（ab7403）/1/ab7403

Abcam/链霉亲和素（HRP）（ab7403）/1/ab7403

价格
面议
货号：ab7403
浏览量：127
品牌：Abcam
服务
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商品描述

Description

  • Product name

    Streptavidin (HRP)See all Streptavidin proteins and peptides

  • Biological activity

    Binds to Biotin. Dissociation constant has not been measured.

  • Expression system

    Native

  • Accession

    P22629

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Streptomyces avidinii

  • Conjugation

    HRP

  • Description

    Native Streptavidin protein (HRP)

Associated products

  • Substrate reagent

    • ABTS™, Peroxidase substrate (ab142041)

Specifications

Our Abpromise guarantee covers the use ofab7403in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

    Immunocytochemistry

    Western blot

  • Form

    Liquid

  • Additional notes

    Horseradishperoxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.

    This product has been assayed against 1.0 µg of Biotinylated IgG in a standard capture ELISA using a peroxidase substrate as ABTS ab142041 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

    Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: 0.01% Gentamicin sulphateConstituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, BSA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all

  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

  • Cellular localization

    Cytoplasmic
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