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蚂蚁淘在线 / 品牌中心 / Abcam / Abcam/Human MRP1 ELISA Kit (ABCC1) (ab272211)/1 x 96 tests/ABCC1) (ab272211

Abcam/Human MRP1 ELISA Kit (ABCC1) (ab272211)/1 x 96 tests/ABCC1) (ab272211

价格
面议
货号：ABCC1)(ab272211
浏览量：127
品牌：Abcam
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商品描述

Overview

  • Product name

    Human MRP1 ELISA Kit (ABCC1)See all MRP1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    SamplenMeanSDCV%
    Extract82.9%

  • Sample type

    Tissue Extracts, Cell Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.151 ng/ml

  • Range

    0.63 ng/ml -40 ng/ml

  • Recovery

    Sample specific recovery
    Sample typeAverage %Range
    Tissue Extracts9594%- 96%
    Cell Lysate9491%- 97%

  • Assay time

    1h30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with:Human

  • Product overview

    HumanMRP1 ELISA kit (ab272211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement ofMRP1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. QuantitateHumanMRP1 with 151 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

    -Single-wash protocol reduces assay time to 90 minutes or less

    -High sensitivity, specificity and reproducibility from superior antibodies

    -Fully validated in biological samples

    -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    MRP1 (Multidrug Resistance-associated Protein 1), also known as ABCC1 (ATP-Binding Cassette sub-family C member 1), mediates export of organic anions and drugs from the cytoplasm. It mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. MRP1 confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components1 x 96 tests
    10X Human MRP1 (ABCC1) Capture Antibody1 x 600µl
    10X Human MRP1 (ABCC1) Detector Antibody1 x 600µl
    10X Wash Buffer PT (ab206977)1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970)1 x 10ml
    Antibody Diluent 4BI1 x 6ml
    Human MRP1 (ABCC1) Lyophilized Recombinant Protein2 vials
    Plate Seals1 unit
    Sample Diluent NS(ab193972)1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
    Stop Solution1 x 12ml
    TMBDevelopment Solution1 x 12ml

  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Drug resistance
    • MRP-related proteins
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Sodium/ion transport
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease

  • Function

    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.

  • Tissue specificity

    Lung, testis and peripheral blood mononuclear cells.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.Contains 2 ABC transmembrane type-1 domains.Contains 2 ABC transporter domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • ABC 29
    • ABC29
    • ABCC
    • ABCC 1
    • Abcc1
    • ATP binding cassette sub family C (CFTR/MRP) member 1
    • ATP binding cassette sub-family C member 1
    • ATP binding cassette subfamily C member 1
    • ATP binding cassette transporter variant ABCC1delta ex13
    • ATP binding cassette transporter variant ABCC1delta ex13&14
    • ATP binding cassette transporter variant ABCC1delta ex25
    • ATP binding cassette transporter variant ABCC1delta ex25&26
    • ATP binding cassette, sub-family C (CFTR/MRP), member 1
    • ATP-binding cassette sub-family C member 1
    • DKFZp686N04233
    • DKFZp781G125
    • GS X
    • GSX
    • Leukotriene C(4) transporter
    • LTC4 transporter
    • MRP
    • MRP 1
    • MRP1
    • MRP1_HUMAN
    • Multidrug resistance associated protein 1
    • Multidrug resistance protein
    • Multidrug resistance-associated protein 1
    • Multiple drug resistance associated protein
    • Multiple drug resistance protein 1
    see all

  • Database links

    • Entrez Gene:4363 Human
    • Omim:158343 Human
    • SwissProt:P33527 Human
    • Unigene:391464 Human
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