Product Description

Recombinant 2019-nCoV NSP7 is produced by our E.coli expression system and the target gene encoding Ser1-Gln83 is expressed with a 6His tag at the C-terminus.Background:The ∼30kb positive-stranded RNA genome of coronaviruses encodes a replication/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps). The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. SARS-CoV 2 nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.Formulation:Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.Purity:> 95 % as determined by reducing SDS-PAGE.Endotoxin:< 1.0 EU per µg as determined by the LAL method.Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. Avoid freeze-thaw cycles.

More Details:Sequence:Ser1-Gln83Fusion Tag:C-6HisAccession:YP_009725303.1Species:VirusExpression System:E. coliMol Mass:12.3 kDaAP Mol Mass:12 kDaSynonyms:SARS-CoV 2 nsp7Datasheet

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.Manufactured by: Elabscience

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Iwai-Chem我们的大肠杆菌表达系统产生了重组的2019-nCoV 3C样蛋白酶，编码Ser1-Gln306的靶基因在N端表达了6His标签。背景：病毒主要蛋白酶（M pro，也称为3CL pro），控制冠状病毒复制复合物的活性。它起半胱氨酸蛋白酶的作用，参与病毒前体多蛋白的蛋白水解切割成冠状病毒复制所需的一系列功能蛋白，被认为是设计抗SARS药物的诱人靶标。配方：  以0.2μm的PBS，1mM EDTA，10％甘油，pH 7.4的过滤溶液形式提供。纯度：  > 95％（通过还原SDS-PAGE确定）。内毒素： 通过LAL方法测定，每µg <1.0 EU。运输：  本产品以液体形式提供。它以蓝色冰/凝胶包装在冷冻温度下运输。收到后立即将其储存在<-20°C下。避免冻融循环。