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商品描述
| D-Glucose | No Glucose |
|---|---|
| L-Glutamine | No L-Glutamine |
| HEPES | No HEPES |
| Sodium Pyruvate | 110.04 mg/L |
| Phenol Red | Phenol Red |
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.
Gendepot在脊椎动物中，已经鉴定出3个主要的肌动蛋白同种型，即α，β和γ。α肌动蛋白存在于肌肉组织中，是收缩装置的主要组成部分。在大多数细胞类型中，β和γ肌动蛋白作为细胞骨架的成分和内部细胞运动的介质共存。β肌动蛋白是高度保守的蛋白，参与细胞运动，结构和完整性。β肌动蛋白是细胞质蛋白。抗肌动蛋白是一种上样对照抗体，对于正确理解您的蛋白质印迹至关重要。β-肌动蛋白加载控制抗体用于通过确认整个凝胶上的蛋白加载均匀来标准化检测到的蛋白水平。 参考文献（0） MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
