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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/Bradford(Coomassie) Protein Assay Plus Reagents, P7201/P7201-050-450ml
商品描述
| Maximum concentrations that are compatible | Amino acids : 1mMAmmonium sulfate : 1MAmpholytes : 0.5%Ascorbic acid :50mMBoric acid : 1mMBrij® 35 : 0.06%CHAPS : 0.5%CHAPSO : 0.5%Citrate : 0.05%Cysteine : 10mMDeoxycholate : 0.1%DMSO : 10%DNA : 1mg/ml |
|---|---|
| Maximum concentrations that are compatible | DTT : 1MEDTA : 100mMEGTA : 50mMEthanol : 10%Glucose : 1MGlycerol : 10%Glycine : 0.1MGuanidine.HCl : 6MHEPES : 0.1M2-mercaptoethanol : 1MMethanol : 10%MES : 0.7MNonidet® P-40 : 0.5% |
| Maximum concentrations that are compatible | Phenol : 5%Sodium azide : 0.5%Sodium chloride : 6MSodium dodecyl sulfate (SDS) : 0.015%Sodium hydroxide : 0.1MSodium phosphate : 0.1MSucrose : 25%Tris : 2MTriton® X-100 : 0.06%tRNA : 0.35mg/mlTween® 20 : 0.03%Urea : 3M |
Bradford(Coomassie) Protein Assay Plus Reagent is designed for rapid and accurate estimation of total protein concentration in solution. The bradford (Coomassie) protein assay relies on the formation of complexes between coomassie brilliant Blue G-250 dye and proteins in solution. This binding causesa shift in the absorption maximum of the dye from 465 nm to 595 nm. The concentration of the protein sample is determined by referencing the absorption to a series of standard protein dilutions assayed in parallel.
The quantification procedure is simple and rapid. The protein sample is mixed with the reagent at room temperature, the absorbance of the solutions is measured at 595 nm. The absorption at 595nm is proportional to the amount of protein present in the solution. The dye binding process is complete inapproximately 5 min with color stability for 1 hour. The working range of the Bradford(Coomassie) Protein Assay Plus Reagent is 1 ~ 2000 ug/ml of protein.
