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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/ProteinSeeker Mammalian Cell Lysis Solution , P9400/P9400-010-100 ml
商品描述
| High yield | Total protein extraction from cultured cells and tissue samples. |
|---|---|
| Fast and Simple | No sonication or freeze-thaw cycles |
| Convenient | room temperature process |
| Compatible with Downstream Applications | - 1D and 2D electrophoresis- Western blotting and immunoprecipitation- Affinity Purification- EMSA, reporter gene assays and enzyme activity |
ProteinSeekerMammalian Cell Lysis Solution is a ready-to-use solution designed for extraction of native, non-denatured proteins from mammalian cultured cells or tissue samples.The procedure, which is based on lysis with mild detergents at room temperature, is fast and simple: no need for sonication, freeze thaw cycles or scraping of adherent cells. The procedure yields proteins in their native, non-denatured functional state. Therefore, isolated proteins can be used directly in many proteomic and enzymatic activity applications, including immunoprecipitation, affinity purification, reporter gene assays and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compatible with quantification assays such as Bradford and Lowry.
Gendepot在脊椎动物中，已经鉴定出3个主要的肌动蛋白同种型，即α，β和γ。α肌动蛋白存在于肌肉组织中，是收缩装置的主要组成部分。在大多数细胞类型中，β和γ肌动蛋白作为细胞骨架的成分和内部细胞运动的介质共存。β肌动蛋白是高度保守的蛋白，参与细胞运动，结构和完整性。β肌动蛋白是细胞质蛋白。抗肌动蛋白是一种上样对照抗体，对于正确理解您的蛋白质印迹至关重要。β-肌动蛋白加载控制抗体用于通过确认整个凝胶上的蛋白加载均匀来标准化检测到的蛋白水平。 参考文献（0） 
