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商品描述
| IC50 | 6.7 nM / 10 nM |
|---|---|
| Target | GSK-3β / GSK-3α |
| Solubility | 23.3mg/mL in DMSO |
CHIR 99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR 99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR 99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM.In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR 99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR 99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ).Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR 99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR 99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR 99021 treatment.
Gendepot在脊椎动物中，已经鉴定出3个主要的肌动蛋白同种型，即α，β和γ。α肌动蛋白存在于肌肉组织中，是收缩装置的主要组成部分。在大多数细胞类型中，β和γ肌动蛋白作为细胞骨架的成分和内部细胞运动的介质共存。β肌动蛋白是高度保守的蛋白，参与细胞运动，结构和完整性。β肌动蛋白是细胞质蛋白。抗肌动蛋白是一种上样对照抗体，对于正确理解您的蛋白质印迹至关重要。β-肌动蛋白加载控制抗体用于通过确认整个凝胶上的蛋白加载均匀来标准化检测到的蛋白水平。 参考文献（0） 
