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公司地址
苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
| Unit Definition | One Weiss unit of T4 DNA Ligase converts 1 nmole of p32 from pyrophosphate into Norit-adsorbable material in 20 minutes at 37℃ in 33 mM Tris-acetate (pH 7.8), 66 mM potassium acetate, 10 mM magnesium acetate, 0.5 mM DTT, and 1 mM ATP. One Weiss unit equals approximately 200 cohesive- end ligation units. |
|---|---|
| Quality Control | T4 DNA Ligase is functionally tested in cloning assays and is free of detectable contaminating DNA exo- and endo-nuclease and RNase activities. |
| Concentration | 1 Weiss unit/㎕ |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 0.1% Triton X-100, and 1 mM DTT, 50% Glycerol |
| 10X Reaction buffer | composed of 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH 7.8 at 25°C). |
| Application | Cloning of restriction fragments |
| Joining linkers and adapters to blunt-ended DNA |
T4 DNA Ligase is the most versatile and commonly used ligase for DNA cloning. This ATP-dependent enzyme covalently joins 5'-phosphates to 3'-hydroxy-lated termini at the blunt or compatible cohesive ends of double-stranded DNA fragments produced by restriction enzyme digestion or other enzymatic processes.
T4 DNA Ligase has no activity on single- stranded nucleic acids. Following a ligation reaction, T4 DNA Ligase may be inactivated by incubation at 65℃ for 10 minutes.
