GenDEPOTProteinG-Agaroseisanaffinitychromatographymediumdesignedforeasy,one-steppurificationofclasses,subclassesandfragmentsofimmunoglobulinsfromBIOLOGicalfluidsandfromcellculturemedia.TherecombinantproteinGligandiscoupledto4%highlycross-linkedagarose.ThestaticbindingcapacityofProteinG-Agaroseisgreaterthan20mgsheepIgG/mlsettledresin.Thedynamicbindingcapacitywillvarydependingonseveralfactorssuchastargetantibody,flowrateetc.ProteinG,abacterialcellwallproteinisolatedfromgroupGStreptococci,bindstomammalianIgGsmainlythroughFcregions.NativeproteinGhas3IgGbindingdomainsandalsositesforalbuminandcellsurfacebinding.ThelatterhavebeeneliminatedfromrecombinantproteinGtoreducenonspecificbinding.AlthoughproteinGhasverysimilartertiarystructurestoproteinA,theiraminoacidcompositionsdiffersignificantly,resultingindifferentbindingcharacteristics.ProteinGcanbeusedforpurificationofmammalianmonoclonalandpolyclonalIgGsthatdonotbindwelltoproteinA.
ProteinGhasgreateraffinitythanproteinAformostmammalianIgGs,especiallyforcertainsubclassesincludinghumanIgG3,mouseIgG1andratIgG2a.UnlikeproteinA,proteinGdoesnotbindtohumanIgM,IgDandIgA.
