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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
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https://www.ebiomall.com
商品描述
| High Contrast | This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain |
|---|---|
| Stain Color | The resulting stain is a darker brown than standard DAB and somewhat more sensitive. |
This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain. The resulting stain isa darker brown than standard DAB and somewhat more sensitive. GenDEPOT recommends using Hematoxyling for Automation for optimal contrast. 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting.
When in the presence of peroxidase enzyme,DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate (High Contrast). The standard working dilution is 50ul (0.9mg) of DAB Chromogen per 1ml of DAB Substrate (High Contrast), although the ratio can be adjusted as desired.
The use of liquid components reduces some risks associated with handling powders (ie. dust inhalation), and eliminates waste which often results from using tablets that require a predetermined final volume. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.
