Gendepot/MTT分析试剂,即用型,M0200/2x1 ml/M0200-002

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¥700.00
货号:M0200-002
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品牌:Gendepot
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商品描述
The MTT Assay Reagent provides a simple method for determining live cellnumbers using a standard colorimetric plate readers. Determination of live cellnumbers is often used to assess the rate of cell proliferation and cytotoxicitycaused by drugs and cytotoxic agents. Among all non-radioactive viability assays,MTT assay developed by Mossman is one of the most versatile and popular assays.
MTT is a tetrazolium salt that is turned into a purple formazan product afterreduction by mitochondrial enzymes that are only present in metabolicallyactive live cells, not in dead cells. The amount of formazan product generatedis proportional to the number of living cells in the sample. At the end of the assay,the cells containing the formazan product are solubilized and then photometricallyquantified at 570 nm.
Gendepot在脊椎动物中,已经鉴定出3个主要的肌动蛋白同种型,即α,β和γ。α肌动蛋白存在于肌肉组织中,是收缩装置的主要组成部分。在大多数细胞类型中,β和γ肌动蛋白作为细胞骨架的成分和内部细胞运动的介质共存。β肌动蛋白是高度保守的蛋白,参与细胞运动,结构和完整性。β肌动蛋白是细胞质蛋白。抗肌动蛋白是一种上样对照抗体,对于正确理解您的蛋白质印迹至关重要。β-肌动蛋白加载控制抗体用于通过确认整个凝胶上的蛋白加载均匀来标准化检测到的蛋白水平。 参考文献(0)