TheperformanceofBetaineEnhancerSolutionissuperiorcomparedtostandardenhancerssuchasformamide,DMSO,TMAC,BSAandnon-ionicdetergentsbecauseofthefollowingeffects:BetainebindspreferentiallytoATrichsequencesinthemajorgroove,therebystABIlizingATrichregionsoftheDNA.BecauseATforms2hydrogenbondsandGCforms3,thebondingofATislessstablethantheoneofGC.AsaconsequenceofthestabilizingeffectofBetaineonATbonding,thestabilityofATbondingandGCbondingisbroughtclosetoanequallevel.
Atthesametime,BetainehasasequenceindependentdestabilizingeffectonallDNA.TheTmofATrichandGCrichsequencesareequalizedandtheoverallTmislowered.
FurThermore,BetaineaidstheprocessofthermostablepolymerasesandreducespausesinpolymerizationcausedbysecondarystructurethatcaninducethepolymerasetodisassociatefromtheDNAstrand.
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| OptimalConcentration | 0.8-1.6MimprovestheamplificationofDNAbyreducingtheformationofsecondarystructureinGC-richregions |
|---|---|
| Application | PCR |