Catalog Number:  10147

Product Name:  Gαi2 Protein Q205L mutant

Synonyms:  Guanine nucleotide binding protein, alphainhibiting activity polypeptide 2, GNAI2, Galphai2

Source:   Human, recombinant full length, His6-tag

Expression Host:   E. coli

Molecular Weight:   40 kDa

Purity:   >95% by SDS-PAGE

Introduction:   Heterotrimeric G proteins arecritical cellular signal transducers. Gαi represents onesub-family of G proteins that could mediate the inhibition ofadenylyl cyclases. Other biochemical and physiological functions ofGαi proteins are being explored.

Amino Acid Sequence   (1-355, Q205L)

MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECR QYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWAD HGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGG LRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Properties

Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl

Physical Appearance (form):  White or clear

Concentration:  1 mg/mL

Storage:   -80°C

Preparation Instructions:  Centrifuge the vial before open the cap and reconstitute in water.Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solutionto protect the protein is recommended and using of non-ionicdetergents such as n-Dodecyl β-D-maltoside (DoDM) orpolyethylene detergents (e.g. C12E10) also help to stabilize theprotein. Avoid repeated freezing and thawing after reconstitution.The purity of His-tagged Gαi2 Q205L was determined by SDS-PAGEand Coomassie Brilliant Blue Staining.

References:  1. Blatt, C. et al., Proc. Nat. Acad. Sci. 85: 7642-7646, 1988.2. Bloch, D. B. et al., Am. J. Hum. Genet. 42: 884-888, 1988.3. Bray, P. et al., Proc. Nat. Acad. Sci. 84: 5115-5119, 1987.4. Itoh, H. et al., J. Biol. Chem. 263: 6656-6664, 1988.5. Lan, K.-L. et al., J. Biol. Chem. 273: 12794-12797, 1998.6. Neer, E. J. et al., Hum. Genet. 77: 259-262, 1987.7. Ogden, S. K. et al., Nature 456: 967-970, 2008.

Datasheet:

Publications:

1.  Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells    Biochem J. 2013 Feb 15;450(1):209-19

2.  CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor    J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61

3.  CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma    Cancer Res. 2012 Jan 1;72(1):122-32

4.  Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts    Endocrinology. 2012 Oct;153(10):4918-28

5.  WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins    Cell Signal. 2011 Mar;23(3):550-4

6.  Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis    Biochim Biophys Acta. 2011 Mar;1813(3):466-72

7.  Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I    SteroidsVolume 77, Issue 5, April 2012, Pages 424–432

8.  Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits    J Biol Chem. 2010 Aug 27;285(35):26976-86

9.  Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7    Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32

10.  Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin    Mol Biol Cell. 2014 Nov 5;25(22):3654-71

11.  Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan    Mol Cell Biol. 2014 Nov 15;34(22):4186-99

12.  Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the BrainJ Biol Chem. 2014 May 2;289(18):12748-58

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