Catalog Number:  26287

Gene Symbol:  EGFR/HER1/ERBB1

Description:   Anti-EGFR (L747-P753) S Mouse Monoclonal Antibody

Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.

Applications:   ELISA, WB, IF, IHC

Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes EGFR (L747-P753) S, but not wild type EGFR protein from vertebrates.

Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant EGFR(L747-P753) S and wildtype proteins. Purified His-tagged EGFR (L747-P753) S protein (702-987) and wild-type protein (702-987) were blotted with anti- EGFR (L747-P753) S mouse antibody (Cat. #26287).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (L747-P753) S antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (L747-P753) S plasmid (right column), then fixed and stained with anti- EGFR (L747-P753) S monoclonal antibody (Cat. #26287).

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Publications:

1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma    Tumor Biology. October 2014

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