CatalogNumber:  21280

GeneSymbol:  ALK,CD246,NBLST3

Description:  Anti-ALKRabbitPolyclonalAntibody

Background:  Anaplasticlymphomakinase(ALK)alsoknownasALKtyrosinekinasereceptororCD246(clusterofdifferentiation246)isaneuronalorphanreceptortyrosinekinase.ALKisessentiallyandtransientlyexpressedinspecificregionsofthecentralandperipheralnervoussystemsandplaysanimportantroleinthegenesisanddifferentiationofthenervoussystem.AbnormalALK,byfusionwithothergenes,gainingadditionalgenecopiesormutations,canbeoncogenic.

Immunogen:  PurifiedALKprotein,humanorigin.

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200

Concentration:  0.2mg/ml

Host:  Rabbit

Format:  Liquid

Clonality:  Polyclonal

Isotype:

Purity:  Purifiedfromserum

Storagebuffer:

Preservative:  no

Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:  RecognizesALKofvertebrates.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

Westernblot:

WesternblotanalysisofrecombinantALKprotein.PurifiedHis-taggedALKproteinwasblottedwithanti-ALKrabbitpolyclonalantibody(Cat.#21280).

Immunohistochemistry:

Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancertissuewithanti-ALKpolyclonalantibody(Cat.#21280).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-RabbitIgG(1:50dilution)wasusedassecondaryantibody.

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26226	Anti-ALK(C1156Y)MouseMonoclonalAntibody	100μL	$495

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