CatalogNumber:  21276

GeneSymbol:  ATM

Description:  Anti-ATMRabbitPolyclonalAntibody

Background:  Ataxiatelangiectasiamutated(ATM)isaserine/threonineproteinkinasethatisrecruitedandactivatedbyDNAdouble-strandbreaks.ItphosphorylatesseveralkeyproteinsthatinitiateactivationoftheDNAdamagecheckpoint,leADIngtocellcyclearrest,DNArepairorapoptosis.ActivityofATMproteinisundertightcontrol,andmutationofATMcancausediseasesuchasAtaxiatelangiectasia(AT)andcancers.

Immunogen:  PurifiedATMprotein,humanorigin.

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200

Concentration:  0.2mg/ml

Host:  Rabbit

Format:  Liquid

Clonality:  Polyclonal

Isotype:

Purity:  Purifiedfromserum

Storagebuffer:

Preservative:  no

Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:  RecognizesATMofvertebrates.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

Westernblot:

WesternblotanalysisofrecombinantATMprotein.PurifiedHis-taggedATMproteinwasblottedwithanti-ATMrabbitpolyclonalantibody(Cat.#21276).

Immunohistochemistry:

Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancertissuewithanti-ATMpolyclonalantibody(Cat.#21276).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-mouseIgG(1:50dilution)wasusedassecondaryantibody.

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