NewEastBio蚂蚁淘在线

Background: cAMPisaubiquitoussecondmessengermediatingcellularresponsestovariousexogenousandendogenoussignalingmolecules.cAMPregulatesphysiologicalprocessesbyactivatingproteinkinases,gatingspecificionchannels,modulatingcellularcyclicnucleotideconcentrationsthroughphosphodiesterases,andactivatingEpac(exchangeproteindirectlyactivatedbycAMP).TheconversionofATPtocAMPiscatalyzedbyadenylylcyclases(ACs).ThemajorfamilyofACsinmammalsisthetransmembraneACswhichhavenineisoformsandcouldbeactivatedbyGproteinGsand/orCa²⁺/calmodulin.ThereisalsoonesolubleACwhichcouldbemodulatedbybicarbonateand/orCa²⁺.

Immunogen: cAMP

Applications: ELISA,WB,IHC

RecommendedDilutions: ELISA1:1000-1:5000,WB1:1000-1:2000

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purifiedfromascites

Storagebuffer:

Preservative: no

Constituents: PBS(withoutMg²⁺andCa²⁺),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:

StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

FAQsDatasheet:

TypicalStandardCurves

Sensitivity AcetylatedVersion

MeanODforBo=0.565±0.007

MeanODforStandard#6=0.500±0.006

DeltaOpticalDensity(0-0.08pmol/mL)=0.065

2SD"softheZeroStandard=0.014

Sensitivity=(0.014/0.065)*0.08pmol/mL=17fmol/mL


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定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。

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Publications:

1. Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1 PflugersArch.2012Feb;463(2):257-68

2. Antiarrhythmiceffectofprolongedmorphineexposureisaccompaniedbyalteredmyocardialadenylylcyclasesignalinginrats PharmacolRep.2012;64(2):351-9

3. UncouplingofM1muscarinicreceptor/G-proteininteractionbyamyloidbeta(1-42) Neuropharmacology.2013Apr;67:272-83

4. SubendocardialIncreaseinReactiveOxygenSpeciesProductionAffectsRegionalContractileFunctioninIschemicHeartFailure AntioxidRedoxSignal.2013Mar20;18(9):1009-20

5. Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1 PflugersArch.2012Feb;463(2):257-68

6. Structuralbasisofanthraxedemafactorneutralizationbyaneutralizingantibody BiochemicalandBiophysicalResearchCommunicationsVolume417,Issue1,6January2012,Pages324–329

7. TransgenicrescueofdefectiveCd36enhancesmyocardialadenylylcyclasesignalinginspontaneouslyhypertensiverats PflügersArchiv-EuropeanJournalofPhysiologyOctober2013,Volume465,Issue10,pp1477-1486

8. OpposingHDAC4nuclearfluxesduetophosphorylationbyβadrenergicactivatedPKAorbyactivityorEpacactivatedCaMKIIinskeletalmusclefibres TheJournalofPhysiologyVolume591,Issue14,pages3605–3623,July2013

9. SexisamajordeterminantofneuronaldysfunctioninNeurofibromatosisType1 AnnalsofNeurologyVolume75,Issue2,pages309–316,February2014

10. cAMP–PKAinhibitionofSK3channelreducedbothCa2+entryandcancercellmigrationbyregulationofSK3–Orai1complex PflügersArchiv-EuropeanJournalofPhysiologyOctober2014,Volume466,Issue10,pp1921-1932

11. Immunomodulatingeffectsofcasein-derivedpeptideQEPVLandQEPVonlymphocytesinvitroandinvivo FoodFunct.,2014,5,2061-2069

12. AgoNIST-DependentAnd-IndependentDopamine-1-LikeReceptorSignallingDifferentlyRegulatesDownstreamEffectors FEBSJournalVolume281,Issue21,pages4792–4804,November2014

13. Alterationofvascularreactivityinheartfailure:Roleofphosphodiesterasestype3and4 BritishJournalofPharmacologyVolume171,Issue23,pages5361–5375,December2014

14. Ischemia/Reperfusion-InducedCHOPExpressionPromotesApoptosisandImpairsRenalFunctionRecovery:TheRoleofAcidosisandGPR4 PLoSOne.2014Oct24;9(10):e110944

15. Comparativestudyofsomatostatin-humanserumalbuminfusionproteinsandnaturalsomatostatinonreceptorbinding,internalizationandactivation PLoSOne.2014Feb27;9(2):e89932