CatalogNumber:  21019

GeneSymbol:  CDC42

Description:  Anti-Cdc42ChickenPolyclonalAntibody

Background:  SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs).

Immunogen:  RecombinantfulllengthCdc42protein

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:1:100-1:5000WB:1:100-1:1000IHC:1:100-1:1000

Concentration:  1mg/ml

Host:  Chicken

Format:  Liquid

Clonality:  Polyclonal

Isotype:  IgY

Purity:  Purifiedfromeggyolk

Storagebuffer:

Preservative:  no

Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:  Cdc42antibodyrecognizesCdc42fromvertebrates.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

Westernblot:

WesternblotanalysisofCdc42proteins.HepGwhole-celllysatewasusedforWesternblotwiththechickenpolyclonalanti-Cdc42antibody(cat#21019).

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。

Publications:

1.  VibrioparahaemolyticusEffectorProteinsSuppressInflammasomeActivationbyInterferingwithHostAutophagySignaling    PLoSPathog.2013Jan;9(1):e1003142

2.  Interferongammainducesactinpolymerization,Rac1activationanddownregulatesphagocytosisinhumanmonocyticcells    Cytokine.2012Jan;57(1):158-68

3.  Invadopodiaandrolling-typemotilityarespecificfeaturesofhighlyinvasivep190bcr-ablleukemiccells    EuropeanJournalofCellBIOLOGyVolume91,Issues11–12,November–December2012,Pages978–987

4.  EpithelialjunctionformationrequiresconfinementofCdc42activitybyanovelSH3BP1complex    JCellBiol.2012Aug20;198(4):677-93

5.  PolarizedmigrationoflymphaticendothelialcellsiscriticallydependentonpodoplaninregulationofCdc42    AmJPhysiolLungCellMolPhysiol.2011Jan;300(1):L32-42

6.  TheAarskog-ScottSyndromeProteinFgd1RegulatesPodosomeFormationandExtracellularMatrixRemodelinginTransformingGrowthFactorbeta-StimulatedAorticEndothelialCells    MolCellBiol.2011Nov;31(22):4430-41

7.  MMP-9andCXCL8/IL-8ArePotentialTherapeuticTargetsinEpidermolysisBullosaSimplex    PLoSOne.2013Jul19;8(7):e70123

8.  p21-ActivatedKinase(PAK)RegulatesCytoskeletalReorganizationandDirectionalMigrationinHumanNeutrophils    PLoSOne.2013Sep3;8(9):e73063

9.  TheCdc42GuanineNucleotideExchangeFactorFGD6CoordinatesCellPolarityandEndosomalMembraneRecyclinginOsteoclasts    JBiolChem.2014Jun27;289(26):18347-59

10.  Cdc42InhibitorandUsesThereof    UnitedStatesPatentApplication20140194451

11.  Theα4NicotinicReceptorPromotesCD4+T-CellProliferationandaHelperT-CellImmuneResponse    MolecularPharmacologyJanuary2014vol.85no.150-61