Description

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WholeCellLysate,1mg(2mg/ml)

GeneralNotes:
Growthmedia:DMEMsupplementedwith10%FBS

CelllysatewaspreparedbyhomogenizationinRIPAbuffer(25mMTrisHClpH7.6,150mMNaCl,1%NP-40,1%sodiumdeoxycholate,0.1%SDS)supplementedwith5µg/mlofaprotininand5µg/mlofleupeptin.Celldebriswereremovedbycentrifugation(5minutesat12,000RPM).ConcentrationoftotalproteinwasdeterminedusingBCAproteinassay.

StorageInstructions
Shippedat4°C.Upondeliveryaliquot.Storeat-80°C.Avoidfreeze/thawcycle.

Volume
0.5ml

Concentration
1mgat2mg/ml

Purity
WholeCellLysate

AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 1mgofA204WholeCellLysate(Catalog#8890)
• 1mgofA375WholeCellLysate(Catalog#8891)
• 1mgofA431WholeCellLysate(Catalog#8892)
• 1mgofA549WholeCellLysate(Catalog#8893)
• 1mgofAGSWholeCellLysate(Catalog#8894)
• 1mgofAR42JWholeCellLysate(Catalog#8895)
• 1mgofARPE-19WholeCellLysate(Catalog#8896)
• 1mgofASMCWholeCellLysate(Catalog#8897)
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• 1mgofAstrocyteWholeCellLysate(Catalog#8899)
• 1mgofB16-F10WholeCellLysate(Catalog#8900)
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• 1mgofbEnd-3WholeCellLysate(Catalog#8902)
• 1mgofBeta-TC-6WholeCellLysate(Catalog#8903)
• 1mgofBHK-21WholeCellLysate(Catalog#8904)
• 1mgofBJWholeCellLysate(Catalog#8905)
• 1mgofBMS2WholeCellLysate(Catalog#8906)
• 1mgofBT-20WholeCellLysate(Catalog#8907)
• 1mgofBxPC-3WholeCellLysate(Catalog#8908)
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• 1mgofC6WholeCellLysate(Catalog#8910)
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• 1mgofCaki-1WholeCellLysate(Catalog#8912)
• 1mgofCalu-3WholeCellLysate(Catalog#8913)
• 1mgofCalu-6WholeCellLysate(Catalog#8914)
• 1mgofCapan-1WholeCellLysate(Catalog#8915)
• 1mgofCFPEoWholeCellLysate(Catalog#8916)
• 1mgofCHOWholeCellLysate(Catalog#8917)
• 1mgofCHO-K1WholeCellLysate(Catalog#8918)
• 1mgofChromaffinWholeCellLysate(Catalog#8919)
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• 1mgofKBWholeCellLysate(Catalog#8952)
• 1mgofKeratinocyteWholeCellLysate(Catalog#8953)
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• 1mgofLNCAPWholeCellLysate(Catalog#8955)
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• 1mgofSKNASWholeCellLysate(Catalog#8983)
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• 1mgof3LLWholeCellLysate(Catalog#8995)
• 1mgof3T3L1WholeCellLysate(Catalog#8996)
• 1mgof4T1WholeCellLysate(Catalog#8997)
• 1mgof786-OWholeCellLysate(Catalog#8998)

Altogen生命科学公司致力于研发，生产和销售特定细胞系的转染试剂，用于细胞间生物分子的传递，并通过对转染试剂类型的设计将siRNA和质粒DNA有效地转入不同的细胞系和原代细胞内。 Altogen公司开发的聚合物，脂质体，纳米粒子为基础的转染技术分别针对分子生物学，组合化学，和细胞生物学而分别应用。 Altogen定制服务提供符合GLP要求定制研究服务，包括代稳定的细胞系，细胞银行和冷冻保存，焦磷酸测序，克隆，RNA干扰（RNAi）和基因沉默服务，发展分析，siRNA文库筛选，并转染服务。稳定的肿瘤细胞株和原代细胞的产生，可以是非常昂贵和费时。该公司的细胞培养科学家的细胞株的选择，无论是利息或shRNA表达载体的稳定表达的基因改造。标准的RNAi技术服务，包括设计与合成的siRNA的利益，验证siRNA的沉默效率，siRNA转染条件的优化，使高效的基因沉默细胞系或原代培养细胞的靶基因。 转染培养细胞的瞬时或稳定的引入外源性分子和遗传物质（即RNA或DNA），通常是在生物实验室用来研究基因功能，基因表达的调节，生化映射，突变分析，和蛋白质的生产。科学家利用各种载体分子，这种分子，使质粒DNA（PDNA），信使RNA（mRNA），短干扰RNA（siRNA），小分子RNA（miRNA）的，并进入肿瘤细胞株和原代细胞的蛋白质的基因交付。不幸的是，无单提货的方法或转染试剂，可以适用于所有类型的细胞，细胞的细胞毒性和转染效率显着不同，取决于试剂，协议，并正在利用细胞类型。 Altogen生物系统公司提供超过60种类型的细胞的预优化转染试剂盒。 纳米粒子，脂质和聚合物基ALTOGEN ®在体内转染试剂，使交付功能的RNA和DNA分子在体内。 PEG脂质体在体内输送系统减少由于PEG修饰的先天免疫反应，并提供高效的siRNA转染的DNA，并在体内的蛋白质。 由科学“杂志（2010年12月17日）：PEG脂质体在体内转染试剂盒siRNA的特色 Altogen生物系统功能的特定细胞系转染试剂盒：   公司：www.altogen.com