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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
EZQuant™CellQuantifyingKit:#CQ01
(UsecouponcodeEZQ15for15%off1x5mlsize)
ProductDescription
TheEZQuant™CellQuantifyingKitusesWST-8toconvenientlydeterminethenumberofviablecellsincellproliferationandcytotoxicityassays.Thereagentisreducedbythemetabolicactivityoflivecellsandtheamountofformazanproducedisdirectlyproportionaltothenumberoflivingcells.OnebottleofEZQuant™yields500testsandnopremixingisrequired.UsingEZQuant™forcellproliferationassayshasbeenshowntocorrelatewellwiththe[3H]-thymidineincorporationassay.Inaddition,EZQuant™exhibitsahigherdetectionsensitivitycomparedtoothertetrazoliumsalts(e.g.,MTT,XTT,MTS,orWST-1).
Features
|  |
ClicktoseeourRESULTS.
LatestCitations
- Peters,MollyC.,etal."ANovelPolyamine-TargetedTherapyforBRAFMutantMelanomaTumors."MedicalSciences6.1(2018):3.
| ProductSpecifications | |
| ProductName | EZQuant™CellQuantifyingKit |
| Catalog# | CQ01 |
| Size | 500tests(CQ01)/2500tests(CQ05)/5000tests(CQ10) |
| Shipping | RoomTemperature |
| StorageandStABIlity | Storeateitherroomtemperature(stableupto6months),0-5°C(stableupto1year)or-20°C(stableupto2years).Forfrequentuse,storeat0-5°C.Thawingandfreezingrepeatedlywillcauseabackgroundincreasethatwillinterferewiththeassay. |
| QualityControl | Appearance,blank,andsensitivitytestsareperformedforeachlot.Onlylotsthatpassthefollowingcriteriaareoffered:BlankAbsorbance(460nm)≤0.700SensitivityAbsorbance(460nm)≥1.000 |
| RestrictedUse | ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures. |
| Protocol |  Download |
- CellCountProtocol
- CellProliferation/CytotoxicityProtocol
- InoculatecellsUSPension(100μl/well)ina96-wellplate.Pre-incubatetheplateinahumidifiedincubator(e.g.,at37°C,5%CO2).
- Add10μloftheEZQuant™solutiontoeachwelloftheplate.Becarefulnottointroducebubblestothewells,sincetheyinterferewiththeO.D.reADIng.
- Incubatetheplatefor1-4hoursintheincubator.
- Measuretheabsorbanceat450nmusingamicroplatereader.Tomeasuretheabsorbancelater,add10μlof1%w/vSDSor0.1MHCltoeachwell,covertheplateandstoreitwithprotectionfromlightatroomtemperature.Noabsorbancechangeshouldbeobservedfor24hours.
