TRIReagent®RTisanupgradedversionofthesingle-stepmethodofRNAisolation.TRIReagent®RTisbasedonthephaseseparationmethodusingphenolandguanidinethiocyanate.Thispatent-pendingreagentsubstantiallyimprovesthequalityofisolatedRNA.Unliketheprevioussingle-stepmethods,TRIReagent®RTisolatesRNAthatisfreeofDNAcontamination.ThepurityofRNAwastestedbya35-cyclePCR.Thus,noDNasetreatmentisnecessarytousetheisolatedRNAinRT-PCR.Also,theisolatedRNAsolubilizesmorequicklyandeasilyascomparedwiththeoldersingle-stepmethods.

TRIReagent®RTprovidesareliable,cost-effectiveandefficientmethodofRNAisolation.Itisacomprehensivereagentavailableforanalysisofgeneexpression.TRIReagent®RTisolatesRNAfromawidevarietyofsamplesofhuman,animal,plant,yeast,bacterialandviralorigin.TRIReagent®RTalsocanbeusedtosimultaneouslyisolateRNA,DNAandproteinsfromthesamesample.TheRNAisolationiscompletedinlessthan1hour.InadditiontototalRNA,TRIReagent®RTcanbeusedtoisolatehighandlowmolecularweightRNAasseparatefractions.

TRIReagent®RT(RT111)

$83.00–$646.00

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TRIReagent®RTisusedforRNAisolationfromtissues,pelletedcellsandcellsgrowninmonolayer.Fiftymillilitersissufficienttoprocess50samples,eachcontaining50mgoftissue.Expectedyieldsrangefrom50–700μgofRNApersample,dependinguponthetissuesource.

SKU:RT111Category:RNAIsolation

TRIReagent®RT-LiquidSample(RL311)

$90.00–$701.00

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TRIReagent®RT-LiquidSamplesisusedforcellsUSPensionsandotherliquidsamples.Fiftymillilitersissufficienttoprocess65samplesof0.25mleach.

SKU:RL311Category:RNAIsolation

References

1. Chomczynski,P.andSacchi,N.Single-stepmethodofRNAisolationbyacidguanidiniumthiocyanatephenol-chloroformextraction.Anal.Biochem.162:156-159,1987.
2. Chomczynski,P.,USpatents4,843,155and5,346,994.
3. Chomczynski,P.Areagentforthesingle-stepsimultaneousisolationofRNA,DNAandproteinsfromcellandtissuesamples.BioTechniques15:532-537,1993.