TRIReagent^®isapatentedreagentfortheisolationoftotalRNAorforthesimultaneousisolationofRNA,DNAandproteins.Thereagentisanimprovedversionofthepopularsingle-stepmethodfortotalRNAisolation(1,2,3).Itisamonophasesolutioncontainingphenolandguanidinethiocyanate.TRIReagent®providesareliable,costeffectiveandefficientmethodofRNAisolation.TRIReagent®allowsforacomprehensiveanalysisofgeneexpressioninavarietyofsamplesofhuman,animal,plant,yeast,bacterialandviralorigin.RNAisolationiscompleteinlessthanonehour,andDNAandproteinisolationsinlessthanthreehours.ThreeversionsofTRIReagent®areavailable,eachdesignedforoptimalisolationefficiencyfromcertaintypesofsamples.

TRIReagent®(TR118)

$68.00–$517.00

FullProtocol(PDF)|SDS(PDF)|ViewProductonU.S.PriceList

TRIReagent®isusedforRNAisolationfromtissues,pelletedcellsandcellsgrowninmonolayer.Fiftymillilitersissufficienttoprocess50samples,eachcontaining50–100mgoftissue.Expectedyieldsrangefrom50–700μgofRNApersample,dependinguponthetissuesource.

SKU:TR118Category:RNAIsolation

TRIReagent®BD(TB126)

$74.00–$562.00

FullProtocal(PDF)|SDS(PDF)|ViewProductonU.S.PriceList

TRIReagent®BDisdesignedforusewithwholebloodandplasma.Fiftymillilitersissufficienttoprocess65samplesof0.25mleach.

SKU:TB126Category:RNAIsolation

TRIReagent®LS(TS120)

$74.00–$562.00

FullProtocal(PDF)|SDS(PDF)|ViewProductonU.S.PriceList

TRIReagent®LSisusedforcellsUSPensionsandotherliquidsamples.Fiftymillilitersissufficienttoprocess65samplesof0.25mleach.

SKU:TS120Category:RNAIsolation

References

USPatents4,843,155and5,346,994.

1. Chomczynski,P.andSacchi,N.Single-stepmethodofRNAisolationbyacidguanidiniumthiocyanatephenol-chloroformextraction.Anal.Biochem.162:156-159.1987.
2. Chomczynski,P.Areagentforthesingle-stepsimultaneousisolationofRNA,DNAandproteinsfromcellandtissuesamples.BioTechniques.15:532-537.1993.
3. Chomczynski,P.,Bowser-Finn,R.andSabatini,L.Areagentforthesingle-stepisolationofviralRNAfromhumanserumandbiopsysamples.J.NIHRes.6:83.1994.