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StABIlizeandstoreRNAatAmbientTemperatures
- PreservesRNAatambienttemperatureswithoutdegradation
- EliminatestheneedtostoreRNAinfreezersortoshipondryice
- ConcentratesRNAmoreeffectivelythanothermethods
- CompatIBLewithmostdownstreamapplications
Overview
RNAstable™andRNAstable™LDallowsyoutopreserveandshipyourtotalRNA,mRNA,andmiRNAforupto12yearsatambienttemperatures*.Theyprovideacost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement.Moreover,theyeliminatesamplelossduetofreeze/thawcycles.WhenyouarereadytouseyourRNAsamples,youcanfullyrecovertheminminuteswithoutanyadditionalpurificationsteps.
RNAstableisavailableintwoconvenientformats:
- RNAstable:providedpre-driedin1.5mlmicrofugetubesand96-wellplatestowhichhydratedRNAcanbeadded.
- RNAstableLD:aliquidreagentwhichcanbeaddeddirectlytoRNAsamplesinyourowntubes,multi-wellplates,orothercontainers

ProductData

Figure1:RNAstableprotectstotalRNA,mRNA,miRNA.Samplescanbesafelystoredfor12yearsatambienttemperatureandprotectedfromdegradation.

Figure2:Successfulgeneexpressionanalysisonmicroarray.

Figure3:(A)AliquotsoftotalRNAwerestoredfor29monthsateither-80°CoratroomtemperatureprotectedinRNAstableorunprotected.RNAintegritywasassessedusingagarosegelelectrophoresisandbioanalyzeranalysis(e.g.RINscore).(B)Aliquotsofthestoredsampleswerequantifiedusingthe18SrRNAgeneinaOne-StepRT-PCRreaction.Threesampleswereamplifiedforeachstorageconditionandtheresultsfromthe9reactionsareoverlaidonthegraph.(C)Recoverylevels(pg)fortotalRNAstoredunderidenticalconditions.
Specifications
- Productformats:
- Pre-driedin1.5mlmicrofugetubesand96-wellplates(RNAstable)
- Liquidaliquotsof2mland10ml(RNAstableLD)
- Applicationvolume:100μl
- Applicationamount:0.01to100µg
- Dryingtime:Overnight(laminarflowhood);0.5to1.5hourswithvacuumconcentratorsystem(speedVac®)
- Recoveryvolume:10to100μl
- Recovery:∼99.9%
- DNAstorageatroomtemperature:12yearsacceleratedaging;3.5years“realtime”
Downloads
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