Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:

• Lentivector-based for transfection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.

• PiggyBac Transposon-based* for infection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.

• Pre-built Jurkat cell line to save time and labor

Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.

*Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

相比传统的四环素病毒调控系统，SBI（system biosciences）最新推出的SparQ™ Cumate   调控系统无疑将为很多利用病毒载体做研究的实验者带来福音。这一系统通过不断的病毒转导，实   现严谨调控，强有力的反馈和可检测的表达，其主要用于诱导调控基因和microRNA的表达。这一   系统通过结合在cumate操纵子上的高亲和能力的CymR阻遏物进行调控，通过加入一种可以结合   在CymR上的无毒的小分子化合物Cumate，来解除抑制。这一系统可以反复使用，精确的开关机   制，从而实现严谨的调控，用作时空表达的研究。                       图一：无渗漏调控系统原理图   相比传统的诱导系统，Cumate 具有更低的背景和强劲的表达，如图二所示，相比其他的系统，SparQ比其他系统表达强32倍，且是零渗漏。  同时SBI还可以根据您的需要定制Cumate系统！