Details

Chickenswereimmunizedwithpurified,non-denaturedgoatIgG(containingbothheavyandlightchains)emulsifiedinFreund’sadjuvant.Aftermultipleinjections,eggswerecollectedfromhyperimmunizedhensandtheIgYfractionpurifiedfromtheyolks.Anti-goatIgG’swereaffinity-purifiedoveracolumnconjugatedwithpurifiedgoatIgG(wholeprotein).

Volume:1mL

Concentration:1mg/mL

Clonality:Polyclonal

Form:AffinityPurified

HostSpecies:Chicken

SpeciesReactivity:Goat

Immunogen:IgG

PhysicalState:Liquid

Buffer:Phosphate-bufferedsaline(PBS)(pH7.2),sodiumazide(0.02%,w/v)(asananti-microbialagent).

Specificity:Thereisnodetectablecross-reactivitywithrabbit,humanormouseIgG

QualityControl

Antibodiesweretestedforspecificityusingdouble-immunodiffusionassaysinagarose.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Forlongerstorageperiods,dilutetheantibodieswithsterileglycerolandstoreat-20°C.Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

↑backtotop

二抗 该系列产品特点在于鸡IgY分子与哺乳动物IgG分子截然不同，因此由之制备的二抗不与来源于兔、小鼠或其他哺乳动物的一抗产生交叉反应，特别适用于组织学的免疫双标检测。该系列源于羊的二抗可交联荧光基团/HRP/生物素/琼脂糖，可广泛用于WB，免疫组化/免疫细胞化学及免疫共沉淀研究。