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商品描述
IPTG,Isopropylβ-D-1-thiogalactopyranoside,bindsthelacrepressorandinducesexpressionofβ-galactosidaseinE.coli.IPTGiswidelyusedtoinducetheexpressionofclonedgenesunderthecontrolofthelacoperonandtoscreenblue/whitecolonyinX-galplate.
Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.
Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.
AdditionalInformation
| ProductName: | IPTG |
|---|---|
| AlsoKnownAs: | isopropyl-β-D-thiogalactopyranoside |
| CatalogNo.: | P1010-500 |
| Size: | 5X100g |
| CAS: | 367-93-1 |
| Formula: | C9H18O5S |
| MolecularWeight: | 238.3 |
| Form: | Powder |
| QualityAssurance: | >99%byHPLC,dioxane-free |
| Source: | Synthetic |
| Storage: | EligIBLeforambienttemperatureshipping.Storeat4°Cuponreceiving. |
| PDFDataSheet: | PDFDatasheet |
| Image(s): |  |
| ShippingMethod: | Roomtemperatureshipping |
| References: | J.Sambrook,D.W.Russell,MolecularCloning:ALaboratoryManual,theThirdedition(ColdSpringHarborLaboratoryPress,ColdSpringHarbor,NewYork,A1.27,2001)pp.1.124-1.125. |
ExpressionofGST-UCHL3inBL21(DE3)cellswasinducedbytheadditionof25μM(lane3),100μM(lane4),200μM(lane5)or400μM(lane6)IPTGat370Cfor2hours.Lane2withoutIPTG.
