ubpbio/抗泛素(P4D1)/Y3012/200µg

价格
¥5800.00
货号:Y3012
浏览量:127
品牌:UBPBio
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商品描述
Anti-UbMouseMonoclonalAntibody

AdditionalInformation

Details

Specificity
Recognizesmonoandpolyubiquitinatedproteins,freepolyubiquitinchainsandubiquitininawiderangeofspecies.Thisantibodyrecognizesdifferenttypesofpolyubiqutinchains.
IsotypeMouseIgG1
CloneP4D1
PurificationProteinGaffinitychromatography
ApplicationsWBandIF
WorkingConditionsWB(withECL):1:1000~1:3000dilution
IF:1:500IP:notrecommended
ForbestresultsofWBandIF,andotherassays(e.g.:DotandELISA),pleasedetermineoptimalworkingdilutionbytitrationtest
ReconstitutionCentrifugebrieflytocollectthepowdertothebottomofthetubepriortoreconstitution.Reconstituteeach100μgantibodyinto100μL50%glycerolinmiliQwater.Finalbuffercompositionis50mMPIPES,Ph7.0,1%sucrose,0.5%dextranand50%glycerol.Add0.02%azidetopreventbacterialcotamination.
Images(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
Ubiquitin-Proteasome Biotechnologies成立于2011年,ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶,E1和E2的酶,泛素和泛素衍生物,蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品,ubpbio致力为全球的客户提供更多的帮助。在人类细胞中,有超过1000的蛋白质/酶,参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理,炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。
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ProductName:Anti-Ubiquitin(P4D1)
AlsoKnownAs:Anti-Ub
CatalogNo.:Y3012
Size:200µg
MolecularWeight:~55/25kDa
Species:Recognizesubiquitininawiderangeofspecies
Source:Immunogen:Fulllengthbovineubiquitin
Stock:lyophilizedpowder
Concentration:lyophilizedpowder
QualityAssurance:Guaranteedfordetecting5ngendogenousubiquitinorpolyubiquitinchainsinwholecellortissuelysatesatanantibodydilutionof1:1,000.
Storage:1)Storethelyophilizedantibodydesccatedat-20°C.2)Afterreconstitution,storetheantibodyat-20°C.Aliquotisnotnecessary.Thequalityoftheantibodyisguaranteedforoneyearafterpuchasingifstoredcorrectly.
PDFDataSheet:PDFDatasheet
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
ShippingMethod:Roomtemperatureshipping
References:N/A