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蚂蚁淘在线 / 品牌中心 / UBPBio / ubpbio/多泛素链捕获试剂盒/J4460/1试剂盒

ubpbio/多泛素链捕获试剂盒/J4460/1试剂盒

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￥7600.00
货号：J4460
浏览量：127
品牌：UBPBio
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商品描述
ThiskitisdesignedforenrichmentofpolyubiquitinatedcellularproteinsusingGSTtaggedrecombinantproteinsthatbindlysine48andlysine63polyubiquitinchains.Itcanbeusedtoenrichpolyubiquitinatedproteinsinwholecellortissuelysates.Boundproteinscanbeprecipitatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione,andthenassayedbyim-munoblottingormassspectrometry.

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Description
ThiskitisdesignedforenrichmentofpolyubiquitinatedcellularproteinsusingGSTtaggedrecombinantproteinsthatbindlysine48andlysine63polyubiquitinchains.Itcanbeusedtoenrichpolyubiquitinatedproteinsinwholecellortissuelysates.Boundproteinscanbeprecipitatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione,andthenassayedbyim-munoblottingormassspectrometry.
Components
•GST-S5a(UIM)/GST-TAB2(NZF)Mix(2mg/mleach)..............1ml
•GST(4mg/ml)...........................................................................0.5ml•Iodoacetamine(IAA)..................................................................25mg
•Glutathione(reduced)..............................................................100mg•GlutathioneAgaroseResin(75%slurry)..................................1.0ml
Images:
(Clickimagetoenlarge)
ProductName:PolyubiquitinChainCaptureKit
AlsoKnownAs:N/A
CatalogNo.:J4460
Size:1Kit
MolecularWeight:N/A
Species:N/A
Source:N/A
Stock:Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:Enzyme>95%bySDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
SchematicofpolyUbchainsenrichment

Lane1:GST
Lane2:GST-S5a(UIM)Lane3:GST-TAB2(NZF)
Lane4:GST-S5a(UIM)+GST-TAB2(NZF)Lane5:GST-NEMO(UBAN)
Accordingtoproceduresdescribedabove,5mgwholecelllysatesfromHeLacellsweremixedwith0.2mgGST(lane1),GST-S5a(UIM)(lane2),GST-TAB2(NZF)(lane3),GST-S5a(UIM)+GST-TAB2(NZF)(lane4),orGST-NEMO(UBAN)(lane5).10%eluateswereseparatedbySDS-PAGE,followedbyimmunoblottingofUb,K48-specificUbchainsandGST.
*NoimmunoreactivitywasdetectedinGST-NEMO(UBAN)pulldownassays,presumablybecausethereislittlelinearpolyubiquitinchainsincells.
ShippingMethod:Dryiceshipping
References:N/A
SchematicofpolyUbchainsenrichment

Lane1:GST
Lane2:GST-S5a(UIM)Lane3:GST-TAB2(NZF)
Lane4:GST-S5a(UIM)+GST-TAB2(NZF)Lane5:GST-NEMO(UBAN)
Accordingtoproceduresdescribedabove,5mgwholecelllysatesfromHeLacellsweremixedwith0.2mgGST(lane1),GST-S5a(UIM)(lane2),GST-TAB2(NZF)(lane3),GST-S5a(UIM)+GST-TAB2(NZF)(lane4),orGST-NEMO(UBAN)(lane5).10%eluateswereseparatedbySDS-PAGE,followedbyimmunoblottingofUb,K48-specificUbchainsandGST.
*NoimmunoreactivitywasdetectedinGST-NEMO(UBAN)pulldownassays,presumablybecausethereislittlelinearpolyubiquitinchainsincells.
Ubiquitin-Proteasome Biotechnologies成立于2011年，ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶，E1和E2的酶，泛素和泛素衍生物，蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品，ubpbio致力为全球的客户提供更多的帮助。在人类细胞中，有超过1000的蛋白质/酶，参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理，炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。