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商品描述
GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. GST-TAB2 (NZF) can be precipitated using glutathione resin. After washing, GST-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 10 mM glutathione.
Additional Information
| Product Name: | GST-TAB2 (NZF) | ||
|---|---|---|---|
| Also Known As: | CHTD2; MAP3K7IP2 | ||
| Catalog No.: | I1721 | ||
| Size: | 500 µg | ||
| Molecular Weight: | 7.7 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~80% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_015093.4 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58 2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809 |
Details
GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. GST-TAB2 (NZF) can be precipitated using glutathione resin. After washing, GST-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 10 mM glutathione.
Images: (Click image to enlarge)
Note: Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3 - 4. Use 0.2 M NaOH to adjust pH back to neutral.
Ubiquitin-Proteasome Biotechnologies成立于2011年，ubpbio是由科学家和投资者创立的私营企业。ubpbio致力于为学术界和工业界提供高质量的试剂和其他相关产品。ubpbio产品线包括的高度纯化的蛋白酶，E1和E2的酶，泛素和泛素衍生物，蛋白酶体抑制剂和蛋白酶基板。ubpbio不断开发创新以增加新的产品，ubpbio致力为全球的客户提供更多的帮助。在人类细胞中，有超过1000的蛋白质/酶，参与维持泛素-蛋白酶体系统的正常活动。失调的UPS已发现涉及大量的人类疾病包括癌症的病理生理，炎性疾病和神经退行性疾病。ubpbio致力为客户提供更好的泛素-蛋白酶体系统解决方案。 Images: (Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-TAB2(627-693) |
Note: Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3 - 4. Use 0.2 M NaOH to adjust pH back to neutral.
