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商品描述
6xHiswasfusedontheN-terminusofthelysine63polyubiquitinchainbindingdomainofTAB2encompassingaminoacids627-693.Thisfusionproteincanbeusedforinvitropulldownassaysandforenrichmentofcellularproteinsconjugatedwithlysine63polyubiquitinchainsinwholecellortissuelysates.6xHis-TAB2(NZF)canbeprecipitatedusingNickelresin.Afterwashing,6xHis-TAB2(NZF)anditsboundproteinscanbeelutedbyabuffercontaining200mMimidazole.
AdditionalInformation
| ProductName: | 6xHis-TAB2(NZF) | ||
|---|---|---|---|
| AlsoKnownAs: | CHTD2;MAP3K7IP2 | ||
| CatalogNo.: | I1710 | ||
| Size: | 100µg | ||
| MolecularWeight: | 7.7kDa | ||
| Species: | Human | ||
| Source: | Bacterialrecombinant | ||
| Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol | ||
| Concentration: | Seetubelabel | ||
| QualityAssurance: | ~85%bySDS-PAGE,seedatasheetforgelimage | ||
| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles | ||
| PDFDataSheet: | PDFDatasheet，MSDS | ||
| NCBIRefSeq: | NM_015093.4 | ||
| Image(s): | (Clickimagetoenlarge)
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| ShippingMethod: | Dryiceshipping | ||
| References: | 1.vanWijkSJ,etal.(2013)NatProtoc.8(7):1449-58 2.vanWijkSJ,etal.(2012)MolCell.14;47(5):797-809 |
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| Coomassie-stainedSDS-PAGE Lane1:Molecularweightmarkers Lane2:5µgpurified6xHis-TAB2(NZF) |
