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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Ub-Rhodamine110isaquenched,fluorescentsubstratefordeubiquitinatingenzymes.CleavageoftheamidebondbetweentheC-terminalglycineofubiquitinandrhodamine110resultsinanincreaseinrhodaminefluorescenceat535nm(Exc.485nm).
AdditionalInformation
| ProductName: | Ub-Rhodamine110 | ||||||
|---|---|---|---|---|---|---|---|
| AlsoKnownAs: | N/A | ||||||
| CatalogNo.: | M3020 | ||||||
| Size: | 50µg | ||||||
| MolecularWeight: | 8936DabyMS | ||||||
| Species: | Human | ||||||
| Source: | Synthetic | ||||||
| Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol | ||||||
| Concentration: | N/A | ||||||
| QualityAssurance: | ≥95%byRP-HPLCandSDS-PAGE | ||||||
| Storage: | Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles. | ||||||
| PDFDataSheet: | PDFDatasheet，MSDS | ||||||
| NCBIRefSeq: | N/A | ||||||
| Image(s): | (Clickimagetoenlarge)
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| ShippingMethod: | Dryiceshipping | ||||||
| References: | 1.A.Tiratetal.,(2005)Anal.Biochem.,343,244-255. 2.U.Hassiepinetal.,(2007)Anal.Biochem.,371,201-207. |
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| Coomassie-stainedSDS-PAGE Lane1:Molecularweightmarkers Lane2:Ub-Rhodamine110 |
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| ActivityassayofUb-Rhodamine110(100nM)withvaryingconcentrationsUCH-L3(5-313pM). |
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| Reactionbuffer:20mMTris,pH7.1at37°C,150mMNaCl,2mMDTT.Reactionscontained0.5μMUb-Rhodamine110alone(control,brownline),orwith10nMGST-Usp15(grayline)orwith20nMGST-Usp15(yellowline).Rhodamine110fluorescencewasmonitoredbyusingaplatereaderwithexcitation/emissionfiltersat485/20and530/30nm,respectively. |
