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商品描述
Z-LLE-AMCisafluorogenicsubstrateforthepeptidylglutamylpeptidehydrolyzing(PGPH)activityorthecaspase-likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis50-200µM.ThereleasedAMCcanbedetectedbyafluorimeterorplatereaderatexcitation/emissionwavelengthsof380nm/460nm,respectively.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
AdditionalInformation
| ProductName: | Z-Leu-Leu-Glu-AMC(Z-LLE-AMC) |
|---|---|
| AlsoKnownAs: | Z-LLE-AMC |
| CatalogNo.: | G2101 |
| Size: | 25mg |
| MolecularWeight: | 664.8Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| QualityAssurance: | >98%byHPLCandNMR |
| Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
| PDFDataSheet: | DownloadPDFdatasheet |
| NCBIRefSeq: | N/A |
| Image(s): | No |
| ShippingMethod: | Roomtemperatureshipping |
| References: | 1.ChenP,etal.(1996)Cell86(6),961–972. 2.ArendtCS,etal.(1997)ProcNatlAcadSci94(14),7156–7161.3.ReidlingerJ,etal.(1997)JBiolChem272(40),24899–24905. |
